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Developmental Studies Hybridoma Bank
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Developmental Studies Hybridoma Bank
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Journal: eLife
Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons
doi: 10.7554/eLife.101533
Figure Lengend Snippet: ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
Article Snippet: Antibody ,
Techniques: Expressing, Staining
Journal: eLife
Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons
doi: 10.7554/eLife.101533
Figure Lengend Snippet: ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.
Article Snippet: Antibody ,
Techniques: Expressing, Staining, Immunofluorescence, Marker